Welcome
Here, you can explore the single-cell RNA sequencing (scRNA-Seq) results from wild type (WT) and β2 integrin-deficient (CD18 Knock-out[KO]) gamma delta (γδ) T cells isolated from mice lungs.
The analysis was carried out using the Seurat pipeline for differential expression. Further details can be found in our paper summarized below which you should cite if you use our data.
Beta2 integrins differentially regulate γδ T cell subset thymic development and peripheral maintenance
C. L. McIntyre, L. Monin, T. D. Otto, J. C. Rop, C. S. Goodyear, A. C. Hayday, and V. L. Morrison.
Paper significance statement
γδ T cells reside in barrier tissues and provide immune protection against infection and cancer. Their anti-tumor potential has led to recent advances in the development of γδ T cell immunotherapy. However, our understanding of the basic biology of these cells, including what molecules and pathways control their maintenance within barrier tissues, remains poor. We demonstrate that β2 integrin adhesion molecules play a major role in regulating γδ T cell subset numbers during homeostasis: the loss of β2 integrin expression results in a striking increase in IL17-producing γδ T cells in the lungs and uterus due to enhanced survival. These findings illustrate a novel mechanism of γδ T cell regulation that may have significant implications for immunotherapy development.
The results are displayed in 2 sections
- Differential expression (DE) - Visualization of gene expression in CD18-KO cells compared to that in WT cells using parameters specified in our paper (i.e the first 15 Principal components and 0.15 as the clustering resolution).
- Clustering - Allows flexibility in increasing the number of clusters by increasing the resolution. This results in the sub-division of clusters characterized in our paper into sub-populations.
Key results
